π…πŒΉπŒΊπŒΉπ€πŒ°πŒΉπŒ³πŒΎπŒ°πŒΉ

π†πŒ΄πŒΉπŒ»πŒ°:Proteolytic cleavage of a Dual-GFP fusion FRET-pair.png

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πƒπŒΊπŒ΄πŒΉπ‚πŒ΄πŒΉπŒ½πƒ
English: If the linker is intact, excitation at the absorbance wavelength of CFP (414nm) causes emission by YFP (525nm) due to FRET. If the linker is cleaved by a protease, FRET is abolished and emission is at the CFP wavelength (425nm).
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πŒ±π‚πŒΏπŒ½πŒ½πŒ° πƒπ…πŒ΄πƒ π…πŒ°πŒΏπ‚πƒπ„π…
πŒ±π‰πŒΊπŒ°π‚πŒ΄πŒΉπƒ Thomas Shafee

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